Faculty Members

Research

NAFLD is emerging as an important cause of liver disease in India, with a prevalence of 9% in rural India to 32% in the general population. NAFLD is an umbrella term which describes a range of related and progressive disorders, characterized by insulin resistance. The earliest stage of NAFLD is hepatic steatosis, which is characterized by the deposition of TG as lipid droplets in more than 5% of hepatocytes. Hepatic steatosis can progress to nonalcoholic steatohepatitis (NASH), characterized by hepatocyte injury, inflammation, and/or collagen deposition (fibrosis). NASH, in turn, can progress to cirrhosis, where hepatocytes are replaced by scar tissue composed primarily of type 1 collagen. Between 10 to 29% of individuals with NASH develop cirrhosis within 10 years. Cirrhosis can ultimately progress to hepatocellular carcinoma (4 to 27% of individuals). NAFLD is a complex genomic disorder which is considered as the hepatic manifestation of the metabolic syndrome.

The factors that promote TG deposition in the liver and the transition from steatosis to steatohepatitis and cirrhosis in humans have not been clearly defined. We have identified 6 SNPs in 6 genes in Indians that are associated with the disease, as well as a 28 gene transcriptomic “signature” that changes during progression from mild to advanced stages of the disease. Insulin resistance and increased body weight are other risk factors. Now we want to functionally understand and validate the role of these genes in NAFLD initiation and progression in vivo and in vitro, with an aim to identify possible therapeutic targets.

Projects: Genomic and Epigenomic analyses of Non Alcoholic Fatty Liver Disease (NAFLD) in the Indian population (funded by Dept. of Biotechnology)

CV

Professional Appointments

• 2001- 2006: Postdoctoral Research Associate, Dept. of Human Genetics, Virginia Commonwealth University, under the mentorship of Dr. Joyce A. Lloyd.
• 2006-2007 : Associate Research Scientist, Health Sciences Research, Philip Morris USA
• 2007-2008 : Project leader, Sensory Services and Product Functionality, Product Innovation, Altria Client Services, Altria, USA.
• 2008-2010 : Leader, Functional Molecular Biology, Sensory Sciences and Product Functionality, Altria Client Services, Altria, USA.

Professional Memberships

Life member SBC, Life member ISHG, Life member CCHuge

Awards/Honors

• Senior Research Fellowship, Dept. of Atomic Energy, Govt. of India, 1995-2000, for pursuing Ph.D program at Saha Institute of Nuclear Physics.
• Junior Research Fellowship, Dept. of Atomic Energy, Govt. of India, 1994, for pursuing M.Phil program at Saha Institute of Nuclear Physics.
• CSIR Research Fellowship, Govt. of India, 1994.
• National Scholarship Award, Govt. of India, 1992 (for pursuing postgraduate studies)
• National Scholarship Award, Govt. of India, 1989 (for pursuing undergraduate studies)
• National Scholarship Award, Govt. of India, 1987 (for excellence in school final examination)

Publications

• Chatterjee A, Basu A, Das K, Singh P, Mondal D, Bhattacharya B,Roychoudhury S, Majumder PP, Chowdhury A, Basu P, Hepatic transcriptome signature correlated with HOMA-IR explains early Nonalcoholic Fatty Liver Disease pathogenesis, Annals of Hepatology(2020), doi:https://doi.org/10.1016/j.aohep.2020.06.009
• Roy N, Dasgupta D, Mukhopadhyay I, Chatterjee A, Das K, Bhowmik P, Das S, Basu P, Santra AK, Datta S, Dhali GK, Chowdhury A, Banerjee S. (2016) Genetic Association and Gene-Gene Interaction Reveal Genetic Variations in ADH1B, GSTM1 and MnSOD Independently Confer Risk to Alcoholic Liver Diseases in India. Plos One 2016 Mar 3;11(3):e0149843. doi: 10.1371/journal.pone.0149843.
• Biswas NK, Chandra V, Sarkar-Roy N, Das T, Bhattacharya RN, Tripathy LN, Basu SK, Kumar S, Das S, Chatterjee A, Mukherjee A, Basu P, Maitra A, Chattopadhyay A, Basu A, Dhara S. (2015) Variant allele frequency enrichment analysis in vitro reveals sonic hedgehog pathway to impede sustained temozolomide response in GBM. Scientific Rep. 21;5:7915. doi: 10.1038/srep07915.
• Chatterjee A, Basu A, Chowdhury A, Das K, Sarkar-Roy N, Majumder PP, Basu P. Comparative analyses of genetic risk prediction methods reveal extreme diversity of genetic predisposition to nonalcoholic fatty liver disease (NAFLD) among ethnic populations of India. J Genet. 2015 Mar;94(1):105-13.
• Basu P*. and Majumder PP. (2012) Understanding the Genomics of Psychiatric disorders: The Expanding Horizon. Eastern J. Psychiatry.  15:1-11.
• Meng H, Joyce A, Adkins DE, BASU P, Jia Y, Li G, Sengupta TK, Zedler BK, Murrelle EL and van den Oord E. (2010) A statistical method for eliminating non-variable CpG sites in high-throughput DNA methylation profiling, BMC Bioinformatics. 11: 227.
• BASU P, Lung T, Lemsaddek W, Giang Sargent T, Williams DC Jr, Basu M, Redmond LC, Lingrel JB, Haar JL, Lloyd JA. (2007) EKLF and KLF2 have compensatory roles in embryonic e-globin gene expression and primitive erythropoiesis, Blood. 110:3417-3425.
• Redmond LC, Haar JL, Dumur CI, Archer KJ, BASU P and Lloyd JA. (2007) Comparing genetic profiles of embryonic day 9 (E9) mouse yolk sac erythroid and epithelial cells isolated by microdissection. Blood Cells, Molecules, and Diseases. 38:175.
• BASU P, Lemsaddek W, Giang Sargent T, Lung T, Basu M, Lingrel J, Haar J and Lloyd JA. (2007) EKLF and KLF2 have compensatory roles in embryonic globin gene expression and primitive erythropoiesis. Blood Cells, Molecules, and Diseases. 38:155.
• Chervanak AP, BASU P, Shin M, Redmond LC, Sheng G and Lloyd JA. (2006) Identification, characterization and expression pattern of the chicken EKLF gene. Developmental Dynamics. 235: 1933-1940.
• Redmond LC, Haar JL, Giebel ML, Dumur CI, BASU P, Ware JL and Lloyd JA. (2006) Isolation of Erythroid Cells from the Mouse Embryonic Yolk Sac by Laser Capture Microdissection and Subsequent Microarray Hybridization. Blood Cells, Molecules and Diseases. 37: 27-32.
• BASU P, Morris PE, Haar J, Wani MA, Lingrel JB, Gaensler KML and Lloyd JA. (2005) KLF2 is essential for primitive erythropoiesis and regulates the human and murine embryonic β–like globin gene in vivo, Blood. 106:2566-71.
• Zhang P, BASU P, Redmond LC, Morris PE, Rupon J, Ginder GD and Lloyd JA. (2005) A functional screen for Krüppel-like factors that regulate the human γ–globin gene through the CACCC promoter element, Blood Cells, Molecules and Diseases. 35: 227-35.
• Haar JL, Redmond LC, BASU P, Lloyd JA. (2005) Enrichment of erythroid cells from mouse yolk sac and fetal liver using laser capture microdissection (LCM), The FASEB journal. 19, A804.
• BASU P, Morris P, Wani MA, Lingrel JB and Lloyd JA. (2005) KLF2 is required for normal ε- and βh1-globin expression, Blood Cells, Molecules and Diseases. 34:76.
• BASU P, Sargent TG, Redmond LC, Aisenberg JC, Kransdorf EP, Wang SZ, Ginder GD and Lloyd JA. (2004) Evolutionary conservation of KLF transcription factors and functional conservation of human β-globin gene regulation in chicken, Genomics. 84, 311-319.
• BASU P, Aisenberg J, Giang Sargent T, Wang SZ, Kransdorf E, Ginder GD and Lloyd JA. (2003) The γ-globin CACCC element in the regulation of human γ- to β-globin switching in a chicken model system. Blood Cells, Molecules, and Diseases. 31:129.
• Aisenberg J, BASU P and Lloyd JA. (2003). Regulation of the human γ-globin gene by KLF family members, Blood Cells, Molecules, and Diseases. 31:127.
• Chattopadhyay B, BASU P, Gangopadhyay PK, Mukherjee SC, Sinha KK, Chakraborty A, Roy T, Roychoudhury S, Majumder PP and Bhattacharyya NP. (2003) Variation of CAG repeats and two intragenic polymorphisms at SCA3 locus among Machado-Joseph disease/SCA3 patients and diverse normal populations from eastern India, Acta Neurologica Scandinavica. 108, 407-414.
• BASU P, Majumder PP, Roychoudhury S and Bhattacharyya NP. (2001) Haplotype analysis of genomic polymorphisms in and around the myotonic dystrophy locus in diverse populations of India, Human Genetics. 108, 310-317.
• BASU P, Chattopadhyay B, Gangopadhaya PK, Mukherjee SC, Sinha KK, Das SK Roychoudhury S, Majumder P and Bhattacharyya NP. (2000) Analysis of CAG repeats in SCA1, SCA2, SCA3, SCA6, SCA7 and DRPLA loci among spinocerebellar ataxia patients and distribution of CAG repeats at the SCA2 and SCA6 loci in nine ethnic populations of eastern India, Human Genetics. 106, 597-604.
• BASU P, Gangopadhaya PK, Mukherjee SC, Das SK, Sinha KK and Bhattacharyya NP. (2000) Molecular anatomy of CTG expansion in Myotonin Protein kinase Gene among Myotonic Dystrophy Patients from Eastern India,   Human Mutation. 16, 372.
• Pramanik S, BASU P, Gangopadhaya PK, Sinha KK, Jha DK, Sinha S, Das SK, Maiti BK, Mukherjee SC, Roychoudhury S, Majumder PP and Bhattacharyya NP. (2000) Analysis of CAG and CCG repeats in Huntingtin gene among HD patients and normal populations of India, European Journal of Human Genetics. 8, 678-682.
• Ghosh B, Gangopadhyay PK, Saha S, Basu N, BASU P and Bhattacharyya NP. (2000) Genetic study of adult onset inherited progressive ataxia, JANEI, 5, 51-54.

Lab members

PhD student

Debopriyo Ganguly

Lab Allumni

Dr. Ankita Chatterjee

Dr. Vikas Chandra

Priyadarshi Basu