Charges

SAHAJ SERVICES-RATE CHART (All charges are without GST)
GENOMICS FACILITY
Service Code Service Name Service Description Total cost per sample for Government organizations (INR) Total cost per sample for Non Government organizations (INR) Email
D1104 Nucleic Acid Isolation DNA Extraction from blood (Automated Method, QIAsymphony) 589 618 core_genomics@nibmg.ac.in
D1504 DNA Extraction from tissue (Automated Method, QIAsymphony) 740 777
R1304 Tissue Total RNA Isolation using Qiagen RNAEasy MiniKit (Manual Method) 1,156 1,214
R1205 Cellular Total RNA Isolation using PureLink Trizol Plus RNAEasy MiniKit (Manual Method) 1,020 1,071
B1404 DNA & RNA Extraction from same tissue (Manual Method) 1,770 1,859
R1305 Tissue Total RNA Isolation using PureLink Trizol Plus RNAEasy MiniKit(Manual Method) 1,020 1,071
QCD001 QA/QC DNA QA/QC 557 585
QCR001 QA/QC RNA QA/QC 669 702
D0213101-30X Human Whole Exome Sequencing Human Core Exome Sequencing [Roche based] (30X) using NovaSeq S4 6,848 7,191
D0213101-100X Human Core Exome Sequencing [Roche based] (100X) using NovaSeq S4 11,267 11,830
D0212101-30X Human Core Exome Sequencing [Agilent based] (30X) using NovaSeq S4 12,094 12,699
D0211101-60X Human Core Exome Sequencing [Illumina based] (60X) using NovaSeq S4 10,363 10,881
D0212101-100X Human Core Exome Sequencing [Agilent based] (100X) using NovaSeq S4 16,513 17,338
D0211101-100X Human Core Exome Sequencing [Illumina based] (100X) using NovaSeq S4 13,014 13,664
R0403101-25M mRNA Sequencing mRNA Sequencing [Roche based] (25 million PE) using Novaseq S4 6,639 6,971
R0403101-50M mRNA Sequencing [Roche based] (50 million PE) using Novaseq S4 9,290 9,755
R0303101-50M Human/Mouse/Rat Whole Transcriptome Sequencing Transcriptome Sequencing- Without Ribodepletion [Roche based] (50 million PE) using Novaseq S4 8,805 9,245
R0333101-50M Ribo-depleted Total Transcriptome Sequencing-Human/Mouse/Rat (HMR) [Roche based] (50 million PE) using Novaseq S4 11,537 12,114
R0303101-100M Transcriptome Sequencing- Without Ribodepletion [Roche based] (100 million PE) using Novaseq S4 14,107 14,813
R0333101-100M Ribo-depleted Total Transcriptome Sequencing-Human/Mouse/Rat (HMR) [Roche based] (100 million PE) using Novaseq S4 16,839 17,681
R0331101-50M Ribo-depleted Total Transcriptome Sequencing-Human/Mouse/Rat (HMR) [Illumina based] (50 million PE) using Novaseq S4 24,659 25,892
R0331101-100M Ribo-depleted Total Transcriptome Sequencing-Human/Mouse/Rat (HMR) [Illumina based] (100 million PE) using Novaseq S4 29,961 31,459
R0323101-25M Metatranscriptomic Sequencing Metatranscriptomic Sequencing (25 million PE) using Novaseq S4 9,257 9,720
R0323101-50M Metatranscriptomic Sequencing (50 million PE) using Novaseq S4 11,908 12,503
R0504103-25M Small RNA Sequencing Small RNA Sequencing (25 million SE) using Novaseq SP 28,448 29,870
D0123106-1Gb Whole Genome Sequencing Bacterial Whole Genome Sequencing [Roche based] (1gb data) using NovaSeq SP 4,707 4,942
D0121106-1Gb Bacterial Whole Genome Sequencing [Illumina based] (1gb data) using NovaSeq SP 11,280 11,844
D0111102-30X Human Whole Genome Sequencing (30X) using NovaSeq S4 81,377 85,446
D0111102-60X Human Whole Genome Sequencing (60X) using NovaSeq S4 1,59,692 1,67,677
D0117316-30X Human Whole Genome Sequencing [ONT based] (30X) using PromethION flow cell 1,50,032 1,57,534
D0721106 Metagenomic Sequencing 16s rRNA (v3-v4) Microbiome Sequencing using NovaSeq SP 2,953 3,101
D0721120 16s rRNA (v3-v4) Microbiome Sequencing  using MiSeq 8,581 9,010
D0121106-10M Shotgun Microbiome Sequencing [Illumina] (10M reads) using NovaSeq SP 13,358 14,026
D0123106-10M Shotgun Microbiome Sequencing [Roche] (10M reads) using NovaSeq SP 8,355 8,773
D0601103-50M Epigenomic Sequencing ChIP Sequening (50 million PE) using Novaseq S4 7,974 8,373
D08114 Genotyping Array Infinium GSA 3,971 4,170
D10114 EPIC Methylation  28,304 29,719
D16115 Omni Express 15,116 15,872
R200613-2.5K Single Cell RNA Sequencing Single Cell RNA Sequencing [10X Genomics] (2500 cells/sample; 20,000 reads/cell) using NovaSeq SP 1,44,801 1,52,041
R200613-5K Single Cell RNA Sequencing [10X Genomics] (5000 cells/sample; 20,000 reads/cell) using NovaSeq SP 163452 171625
R2006110-2.5K Single Cell RNA Sequencing [10X Genomics] (2500 cells/sample; 20,000 reads/cell) using NovaSeq S1 1,42,256 1,49,369
R2006110-5K Single Cell RNA Sequencing [10X Genomics] (5000 cells/sample; 20,000 reads/cell) using NovaSeq S1 1,58,362 1,66,280
R2006110-10K Single Cell RNA Sequencing [10X Genomics] (10,000 cells/sample; 20,000 reads/cell) using NovaSeq S1 1,90,573 2,00,102
R200113-2K Single Cell RNA Sequencing [Illumina] (2000 cells/sample; 20,000 reads/cell) using NovaSeq SP 8,32,83 87,447
R200113-10K Single Cell RNA Sequencing [Illumina] (10,000 cells/sample; 20,000 reads/cell) using NovaSeq SP 1,45,453 1,52,726
R2001110-2K Single Cell RNA Sequencing [Illumina] (2000 cells/sample; 20,000 reads/cell) using NovaSeq S1 78,192 82,102
R2001110-10K Single Cell RNA Sequencing [Illumina] (10,000 cells/sample; 20,000 reads/cell) using NovaSeq S1 1,31,878 1,38,472
B18061 Custom Services Illumina NovaSeq Sequencing Please contact for price Please contact for price
B18062 Illumina MiSeq Sequencing Please contact for price Please contact for price
B19061/B19062 Custom Library and Sequencing Please contact for price Please contact for price
CS001 Custom Services Please contact for price Please contact for price
DATA CENTER FACILITY
Service Code Service Name Service Description Total cost per sample for Government organizations (INR) Total cost per sample for Non Government organizations (INR) Email
Analysis service** core_datacenter@nibmg.ac.in
DIARR001 Genotyping array - Genotype calling - upto 500K probes - Illumina Genotype call report - Per sample basis 50 75
DIARR002 Genotyping array - Genotype calling - more than 500K probes - Illumina
Genotype call report - Per sample basis		 250 375
DHOT0001 Hotspot targeted sequening data analysis  - Illumina Annotated Hotspot mutation call - Only SNP/Indel - per sample 500 750
DIEXSP01 Exome sequencing data analysis - Somatic - Paired - Illumina
Annotated Somatic variant calls - Only SNP/Indel - per paired samples		 2,500 3,750
DIEXD002 Exome sequencing data analysis -  Illumina Annotated Germline variant calls - Only SNP/Indel - per sample 1,000 1,500
DIEXST03 Exome sequencing data analysis - Somatic - Tumor only - Illumina Annotated Somatic variant calls - Only SNP/Indel - per sample 1,500 2,250
DIWGSP01 Whole genome sequencing data analysis - Somatic - Paired - Illumina Annotated Somatic variant calls - Only SNP/Indel - per paired sample 7,500 11,250
DIWGD002 Whole genome sequencing data analysis - Illumina Annotated Germline variant calls - Only SNP/Indel - per sample 3,500 5,250
DIRNAS001 mRNAseq analysis (Input Fastq)- Single sample / group - Illumina Gene expression count - per sample 1,000 1,500
DIRNAP002 mRNAseq analysis (Input Fastq) - Paired sample / group - Illumina Differential expression analysis - per group 2,500 3,750
DRNADE03 Differential gene (mRNA) expression annotation analysis - Illumina Pathway analysis - per job 3,000 4,500
Consultation service
DNGSC001 Project consultation on NGS data analysis / study design Per hour of consultation time 5,000 7,500
DSTAT001 Project consultation on statistical data analysis / study design Per hour of consultation time 5,000 7,500
Consulation and analysis
DCUST001 Custom analysis - Based on discussion and approval Per day of custom analysis manpower time 10,000 15,000
Training
DTRAN001 Training of programming, data management, server software installation and data analysis Per month of stay and training in NIBMG, Guest house charges additional as per prevailing rate, maximum two months. 80,000 1,20,000
** includes hardware, software infrastructure, operational costs such as manpower and consultation
ZEBRAFISH FACILITY
Service Code Service Name Service Description Total cost per sample for Government organizations (INR) Total cost per sample for Non Government organizations (INR) Email
ZF2301 Zebrafish facility
Mirco-injections		 1. All needed items for Microinjections
2. Imaging
3. Maintenance and care		 5,000 7,500 core_zebrafish@nibmg.ac.in
Anti-sense oligo/morpholino (one-cell stage) or CRISPR (one-cell stage) or Plasmid (one-cell stage) or Cells (at 2dpf stage) (Needs to provide own reagent and IAEC approved). 4. Deep Phenotypic Study (Cryotome, staining, Slide preparation, imaging) (Including fixations medium) 7,000 10,500
5. Confocal Imaging (per hour) 500 750
ZF2302 Drug Screening 1. Drug treatment
2. Dechorionation
3. Screening and imaging
4. Maintenance and care (Needs to provide own drug and IAEC approved.)		 3,000 4,500
Needs to provide own drug and IAEC approved.
5. Confocal Imaging (per hour) 500 750
ZF2303 Behavioural Study 1. Using ZANTIKS System Analyser
2. Data analysis
3. Maintenance and care		 3,000 4,500
Experimental Conditions at 1dpf -14dpf larvae (IAEC approved)
ZF2304 IAEC approval Protocol submission and execution, fees for IAEC, NIBMG 5,000 7,000
HUMAN MICROBIOME FACILITY
Service Code Service Name Service Description Total cost per sample for Government organizations (INR) Total cost per sample for Non Government organizations (INR) Email
HMF001 Microbiome DNA/RNA isolation (Manual) Minimum number of samples needed per user-50 2,100 3,150 core_humanmicrobiome@nibmg.ac.in
HMF002 Microbiome DNA/RNA isolation (Automated) Minimum number of samples needed per user-192 2,100 3,150
HMF003 16S based PCR and Gel electrophoresis Minimum number of samples needed per user-24 1,600 2,400
HMF004 Project Consultation: Study Design and Data Analysis Per hour consultation time 5,000 7,500
HMF005* 16S data analysis* Minimum number of samples needed per user-10
(Deliverables: OTU/ASV, L2-L6, Core Diversity, Biome file)		 1000 1,500
HMF006* Shotgun data analysis* Based on discussion with facility-in-charge
HMF007 Training in wet lab and dry lab modules
2-3 weeks stay and training in NIBMG, Guest house charges additional as per prevailing rate		 30,000 45,000
* The data analysis services will be carried out jointly with the data centre
BFDU FACILITIES
Service Code Service Name Service Description Total cost per sample for Government organizations (INR) Total cost per sample for Non Government organizations (INR) Email
BFDU001 Bioplex Bioplex use per plate 14,135 20,502 core_bfdu@nibmg.ac.in
BFDU002 FACS analysis FACS Analysis per hour 2,500 3,500
BFDU003 FACS sorting FACS sorting per hour 3,500 5,000
BFDU004 DD-PCR DD PCR per run; reagent kit not included 1,500 2,000
BFDU005 Ultracentrifuge Ultracentrifuge run per hour; tube not included 1,500 2,000
IMAGING FACILITY
Service Code Service Name Service Description Total cost per sample for Government organizations (INR) Total cost per sample for Non Government organizations (INR) Email
CM001 Confocal microscopy Use of confocal microscope facility per hour 500 750 core_imaging@nibmg.ac.in
PROTEOMICS FACILITY
Service Code Service Name Service Description Total cost per sample for Government organizations (INR) Total cost per sample for Non Government organizations (INR) Email
MS001 Protein identification Protein identification from single spot/band 5,000 10,000 core_proteomics@nibmg.ac.in
MS002 Protein complexes (pull down) identification 6,000 12,000
MS003 LC-MS/MS proteome analysis  LC-MS/MS proteome analysis (digested sample) [ORBITRAP MS/MS], Run 180 mins 15,000 30,000
MS004 LC-MS/MS proteome analysis (including sample preparation) [ORBITRAP MS/MS, Run 180 mins 20,000 40,000
For a combination of above mentioned services core_combinationservice@nibmg.ac.in