| SAHAJ SERVICES-RATE CHART (All charges are without GST) | |||||
| GENOMICS FACILITY | |||||
| Service Code | Service Name | Service Description | Total cost per sample for Government organizations (INR) | Total cost per sample for Non Government organizations (INR) | |
| D1104 | Nucleic Acid Isolation | DNA Extraction from blood (Automated Method, QIAsymphony) | 878 | 1097 | core_genomics@nibmg.ac.in |
| D1504 | DNA Extraction from tissue (Automated Method, QIAsymphony) | 886 | 1,107 | ||
| R1304 | Tissue Total RNA Isolation using Qiagen RNAEasy MiniKit (Manual Method) | 1,206 | 1,508 | ||
| R1205 | Cellular Total RNA Isolation using PureLink Trizol Plus RNAEasy MiniKit (Manual Method) | 1,556 | 1,945 | ||
| B1404 | DNA & RNA Extraction from same tissue (Manual Method) | 1,815 | 2,269 | ||
| R1305 | Tissue Total RNA Isolation using PureLink Trizol Plus RNAEasy MiniKit(Manual Method) | 1,564 | 1,955 | ||
| NA | QA/QC | RNA QA/QC | 611 | 764 | |
| D0213101-30X | Human Whole Exome Sequencing | Human Core Exome Sequencing [Roche based] (30X) using NovaSeq S4 | 7,339 | 9,174 | |
| D0213101-100X | Human Core Exome Sequencing [Roche based] (100X) using NovaSeq S4 | 12,116 | 15,145 | ||
| D0212101-30X | Human Core Exome Sequencing [Agilent based] (30X) using NovaSeq S4 | 12,887 | 16,109 | ||
| D0211101-60X | Human Core Exome Sequencing [Illumina based] (60X) using NovaSeq S4 | 17,521 | 21,901 | ||
| D0212101-100X | Human Core Exome Sequencing [Agilent based] (100X) using NovaSeq S4 | 17,664 | 22,080 | ||
| D0211101-100X | Human Core Exome Sequencing [Illumina based] (100X) using NovaSeq S4 | 20,387 | 25,484 | ||
| R0403101-25M | mRNA Sequencing | mRNA Sequencing [Roche based] (25 million PE) using Novaseq S4 | 6,824 | 8,531 | |
| R0403101-50M | mRNA Sequencing [Roche based] (50 million PE) using Novaseq S4 | 9,691 | 12,113 | ||
| R0303101-50M | Human/Mouse/Rat Whole Transcriptome Sequencing | Transcriptome Sequencing- Without Ribodepletion [Roche based] (50 million PE) using Novaseq S4 | 9,291 | 11,614 | |
| R0333101-50M | Ribo-depleted Total Transcriptome Sequencing-Human/Mouse/Rat (HMR) [Roche based] (50 million PE) using Novaseq S4 | 11,367 | 14,209 | ||
| R0303101-100M | Transcriptome Sequencing- Without Ribodepletion [Roche based] (100 million PE) using Novaseq S4 | 15,024 | 18,780 | ||
| R0333101-100M | Ribo-depleted Total Transcriptome Sequencing-Human/Mouse/Rat (HMR) [Roche based] (100 million PE) using Novaseq S4 | 17,100 | 21,375 | ||
| R0331101-50M | Ribo-depleted Total Transcriptome Sequencing-Human/Mouse/Rat (HMR) [Illumina based] (50 million PE) using Novaseq S4 | 24,924 | 31,155 | ||
| R0331101-100M | Ribo-depleted Total Transcriptome Sequencing-Human/Mouse/Rat (HMR) [Illumina based] (100 million PE) using Novaseq S4 | 30,656 | 38,320 | ||
| R0323101-25M | Metartranscriptomic Sequencing | Metatranscriptomic Sequencing [Illumina based] (25 million PE) using Novaseq S4 | 19,161 | 23,952 | |
| R0323101-50M | Metatranscriptomic Sequencing [Illumina based] (50 million PE) using Novaseq S4 | 22,028 | 27,534 | ||
| R0501103-25M | Small RNA Sequencing | Small RNA Sequencing [Illumina based] (25 million SE) using Novaseq SP | 35,105 | 43,881 | |
| R0504103-25M | Small RNA Sequencing [Qiagen based] (25 million SE) using Novaseq SP | 29,741 | 37,176 | ||
| D0123106-1Gb | Whole Genome Sequencing | Bacterial Whole Genome Sequencing [Roche based] (1gb data) using NovaSeq SP | 4,526 | 5,658 | |
| D0121106-1Gb | Bacterial Whole Genome Sequencing [Illumina based] (1gb data) using NovaSeq SP | 11,688 | 14,610 | ||
| D0111102-30X | Human Whole Genome Sequencing (30X) using NovaSeq S4 | 89,951 | 1,12,438 | ||
| D0111102-60X | Human Whole Genome Sequencing (60X) using NovaSeq S4 | 1,70,584 | 2,13,230 | ||
| D0721106 | Metagenomic Sequencing | 16s rRNA (v3-v4) Microbiome Sequencing using NovaSeq SP | 2,953 | 3,691 | |
| D0721120 | 16s rRNA (v3-v4) Microbiome Sequencing using MiSeq | 8,581 | 10,727 | ||
| D0121106-10M | Shotgun Microbiome Sequencing [Illumina] (10M reads) using NovaSeq SP | 14,000 | 17,500 | ||
| D0123106-10M | Shotgun Microbiome Sequencing [Roche] (10M reads) using NovaSeq SP | 8,467 | 10,584 | ||
| D0601103-50M | Epigenomic Sequencing | ChIP Sequening (50M reads) | 29,812 | 37,265 | |
| D08114 | Genotyping Array | Infinium GSA | 4,411 | 5,514 | |
| D09114 | Omni 2.5 | 25,306 | 31,633 | ||
| D10114 | EPIC Methylation | 34,697 | 43,371 | ||
| D16115 | Omni 5.0 | 40,736 | 50,920 | ||
| D1701 | Targeted Sequencing | Targeted Sequencing (custom Panel) | Please contact for price | Please contact for price | |
| B18061 | Sequencing Only | Illumina NovaSeq Sequencing | Please contact for price | Please contact for price | |
| B18062 | Illumina MiSeq Sequencing | Please contact for price | Please contact for price | ||
| B19061/B19062 | Custom Sequencing | Custom Library and Sequencing | Please contact for price | Please contact for price | |
| DATA CENTER FACILITY | |||||
| Service Code | Service Name | Service Description | Total cost per sample for Government organizations (INR) | Total cost per sample for Non Government organizations (INR) | |
| Analysis service** | core_datacenter@nibmg.ac.in | ||||
| DIARR001 | Genotyping array - Genotype calling - upto 500K probes - Illumina | Genotype call report - Per sample basis | 50 | 75 | |
| DIARR002 | Genotyping array - Genotype calling - more than 500K probes - Illumina | Genotype call report - Per sample basis | 250 | 375 | |
| DHOT0001 | Hotspot targeted sequening data analysis - Illumina | Annotated Hotspot mutation call - Only SNP/Indel - per sample | 500 | 750 | |
| DIEXSP01 | Exome sequencing data analysis - Somatic - Paired - Illumina | Annotated Somatic variant calls - Only SNP/Indel - per paired samples | 2,500 | 3,750 | |
| DIEXD002 | Exome sequencing data analysis - Illumina | Annotated Germline variant calls - Only SNP/Indel - per sample | 1,000 | 1,500 | |
| DIEXST03 | Exome sequencing data analysis - Somatic - Tumor only - Illumina | Annotated Somatic variant calls - Only SNP/Indel - per sample | 1,500 | 2,250 | |
| DIWGSP01 | Whole genome sequencing data analysis - Somatic - Paired - Illumina | Annotated Somatic variant calls - Only SNP/Indel - per paired sample | 7,500 | 11,250 | |
| DIWGD002 | Whole genome sequencing data analysis - Illumina | Annotated Germline variant calls - Only SNP/Indel - per sample | 3,500 | 5,250 | |
| DIRNAS001 | mRNAseq analysis (Input Fastq)- Single sample / group - Illumina | Gene expression count - per sample | 1,000 | 1,500 | |
| DIRNAP002 | mRNAseq analysis (Input Fastq) - Paired sample / group - Illumina | Differential expression analysis - per group | 2,500 | 3,750 | |
| DRNADE03 | Differential gene (mRNA) expression annotation analysis - Illumina | Pathway analysis - per job | 3,000 | 4,500 | |
| Consultation service | |||||
| DNGSC001 | Project consultation on NGS data analysis / study design | Per hour of consultation time | 5,000 | 7,500 | |
| DSTAT001 | Project consultation on statistical data analysis / study design | Per hour of consultation time | 5,000 | 7,500 | |
| Consulation and analysis | |||||
| DCUST001 | Custom analysis - Based on discussion and approval | Per day of custom analysis manpower time | 10,000 | 15,000 | |
| Training | |||||
| DTRAN001 | Training of programming, data management, server software installation and data analysis | Per month of stay and training in NIBMG, Guest house charges additional as per prevailing rate, maximum two months. | 80,000 | 1,20,000 | |
| ** includes hardware, software infrastructure, operational costs such as manpower and consultation | |||||
| ZEBRAFISH FACILITY | |||||
| Service Code | Service Name | Service Description | Total cost per sample for Government organizations (INR) | Total cost per sample for Non Government organizations (INR) | |
| ZF2301 | Zebrafish facility Mirco-injections | 1. All needed items for Microinjections 2. Imaging 3. Maintenance and care | 5,000 | 7,500 | core_zebrafish@nibmg.ac.in |
| Anti-sense oligo/morpholino (one-cell stage) or CRISPR (one-cell stage) or Plasmid (one-cell stage) or Cells (at 2dpf stage) (Needs to provide own reagent and IAEC approved). | 4. Deep Phenotypic Study (Cryotome, staining, Slide preparation, imaging) (Including fixations medium) | 7,000 | 10,500 | ||
| 5. Confocal Imaging (per hour) | 500 | 750 | |||
| ZF2302 | Drug Screening | 1. Drug treatment 2. Dechorionation 3. Screening and imaging 4. Maintenance and care (Needs to provide own drug and IAEC approved.) | 3,000 | 4,500 | |
| Needs to provide own drug and IAEC approved. | |||||
| 5. Confocal Imaging (per hour) | 500 | 750 | |||
| ZF2303 | Behavioural Study | 1. Using ZANTIKS System Analyser 2. Data analysis 3. Maintenance and care | 3,000 | 4,500 | |
| Experimental Conditions at 1dpf -14dpf larvae (IAEC approved) | |||||
| ZF2304 | IAEC approval | Protocol submission and execution, fees for IAEC, NIBMG | 5,000 | 7,000 | |
| HUMAN MICROBIOME FACILITY | |||||
| Service Code | Service Name | Service Description | Total cost per sample for Government organizations (INR) | Total cost per sample for Non Government organizations (INR) | |
| HMF001 | Microbiome DNA/RNA isolation (Manual) | Minimum number of samples needed per user-50 | 2,100 | 3,150 | core_humanmicrobiome@nibmg.ac.in |
| HMF002 | Microbiome DNA/RNA isolation (Automated) | Minimum number of samples needed per user-192 | 2,100 | 3,150 | |
| HMF003 | 16S based PCR and Gel electrophoresis | Minimum number of samples needed per user-24 | 1,600 | 2,400 | |
| HMF004 | Project Consultation: Study Design and Data Analysis | Per hour consultation time | 5,000 | 7,500 | |
| HMF005* | 16S data analysis* | Minimum number of samples needed per user-10 (Deliverables: OTU/ASV, L2-L6, Core Diversity, Biome file) | 1000 | 1,500 | |
| HMF006* | Shotgun data analysis* | Based on discussion with facility-in-charge | |||
| HMF007 | Training in wet lab and dry lab modules | 2-3 weeks stay and training in NIBMG, Guest house charges additional as per prevailing rate | 30,000 | 45,000 | |
| * | The data analysis services will be carried out jointly with the data centre | ||||
| BFDU FACILITIES | |||||
| Service Code | Service Name | Service Description | Total cost per sample for Government organizations (INR) | Total cost per sample for Non Government organizations (INR) | |
| BFDU001 | Bioplex | Bioplex use per plate | 14,135 | 20,502 | core_bfdu@nibmg.ac.in |
| BFDU002 | FACS analysis | FACS Analysis per hour | 2,500 | 3,500 | |
| BFDU003 | FACS sorting | FACS sorting per hour | 3,500 | 5,000 | |
| BFDU004 | DD-PCR | DD PCR per run; reagent kit not included | 1,500 | 2,000 | |
| BFDU005 | Ultracentrifuge | Ultracentrifuge run per hour; tube not included | 1,500 | 2,000 | |
| IMAGING FACILITY | |||||
| Service Code | Service Name | Service Description | Total cost per sample for Government organizations (INR) | Total cost per sample for Non Government organizations (INR) | |
| CM001 | Confocal microscopy | Use of confocal microscope facility per hour | 500 | 750 | core_imaging@nibmg.ac.in |
| PROTEOMICS FACILITY | |||||
| Service Code | Service Name | Service Description | Total cost per sample for Government organizations (INR) | Total cost per sample for Non Government organizations (INR) | |
| MS001 | Protein identification | Protein identification from single spot/band | 5,000 | 10,000 | core_proteomics@nibmg.ac.in |
| MS002 | Protein complexes (pull down) identification | 6,000 | 12,000 | ||
| MS003 | LC-MS/MS proteome analysis | LC-MS/MS proteome analysis (digested sample) [ORBITRAP MS/MS], Run 180 mins | 15,000 | 30,000 | |
| MS004 | LC-MS/MS proteome analysis (including sample preparation) [ORBITRAP MS/MS, Run 180 mins | 20,000 | 40,000 | ||
| For a combination of above mentioned services | core_combinationservice@nibmg.ac.in | ||||
