| SAHAJ SERVICES-RATE CHART (All charges are without GST) | |||||
GENOMICS FACILITY | |||||
| Service Code | Service Name | Service Description | Total cost per sample for Government organizations (INR) | Total cost per sample for Non Government organizations (INR) | |
| D1104 | Nucleic Acid Isolation | DNA Extraction from blood (Automated Method, QIAsymphony) | 785 | 1,178 | core_genomics@nibmg.ac.in |
| D1504 | DNA Extraction from tissue (Automated Method, QIAsymphony) | 801 | 1,202 | ||
| R1304 | Tissue Total RNA Isolation using Qiagen RNAEasy MiniKit (Manual Method) | 1,154 | 1,731 | ||
| R1205 | Cellular Total RNA Isolation using PureLink Trizol Plus RNAEasy MiniKit (Manual Method) | 1,556 | 2,334 | ||
| B1404 | DNA & RNA Extraction from same tissue (Manual Method) | 1,856 | 2,784 | ||
| R1305 | Tissue Total RNA Isolation using PureLink Trizol Plus RNAEasy MiniKit(Manual Method) | 2,130 | 3,196 | ||
| D0213101-30X | Human Whole Exome Sequencing | Human Core Exome Sequencing [Roche based] (30X) using NovaSeq S4 | 11,525 | 17,287 | |
| D0212101-30X | Human Core Exome Sequencing [Agilent based] (30X) using NovaSeq S4 | 12,840 | 19,260 | ||
| D0213101-100X | Human Core Exome Sequencing [Roche based] (100X) using NovaSeq S4 | 16,206 | 24,309 | ||
| D0211101-60X | Human Core Exome Sequencing [Illumina based] (60X) using NovaSeq S4 | 17,071 | 25,606 | ||
| D0212101-100X | Human Core Exome Sequencing [Agilent based] (100X) using NovaSeq S4 | 17,521 | 26,282 | ||
| D0211101-100X | Human Core Exome Sequencing [Illumina based] (100X) using NovaSeq S4 | 19,880 | 29,820 | ||
| R0333101-50M | Human/Mouse/Rat/Bacteria Whole Transcriptome Sequencing | Ribo-depleted Total Transcriptome Sequencing-Human/Mouse/Rat [Roche based] (50Million Paired-End Reads) using NovaSeq S4 | 14,637 | 21,955 | |
| R0333108-50M | Ribo-depleted Total Transcriptome Sequencing-Human/Mouse/Rat [Roche based] (50Million Paired-End Reads) using NovaSeq S2 | 18,744 | 28,117 | ||
| R0333101-100M | Ribo-depleted Total Transcriptome Sequencing-Human/Mouse/Rat [Roche based] (100M PE)using NovaSeq S4 | 20,255 | 30,382 | ||
| R0321101-50M | Ribo-depleted Total Transcriptome Sequencing-Bacteria [Illumina based] (100M PE) using NovaSeq S4 | 20,670 | 31,005 | ||
| R0331101-50M | Ribo-depleted Total Transcriptome Sequencing-Human/Mouse/Rat [Illumina based] (50M PE) using NovaSeq S4 | 24,233 | 36,349 | ||
| R0321108-50M | Ribo-depleted Total Transcriptome Sequencing-Bacteria [Illumina based] (100M PE) using NovaSeq S2 | 24,778 | 37,167 | ||
| R0331108-50M | Ribo-depleted Total Transcriptome Sequencing-Human/Mouse/Rat [Illumina based] (50M PE) using NovaSeq S2 | 28,340 | 42,511 | ||
| R0333108-100M | Ribo-depleted Total Transcriptome Sequencing-Human/Mouse/Rat [Roche based] (100M PE) using NovaSeq S2 | 28,470 | 42,705 | ||
| R0331101-100M | Ribo-depleted Total Transcriptome Sequencing-Human/Mouse/Rat [Illumina based] (100M PE) using NovaSeq S4 | 29,851 | 44,776 | ||
| R0331108-100M | Ribo-depleted Total Transcriptome Sequencing-Human/Mouse/Rat [Illumina based] (100M PE) using NovaSeq S2 | 38,066 | 57,099 | ||
| R0501103-25M | Small RNA Sequencing | Small RNA Sequencing (25 M reads) | 30,142 | 45,214 | |
| R0403101-50M | mRNA Sequencing | mRNA Sequencing [Roche based] (50Million Paired-End Reads) using NovaSeq S4 | 11,865 | 17,797 | |
| R0403108-50M | mRNA Sequencing [Roche based] (50Million Paired-End Reads) using NovaSeq S2 | 15,973 | 23,959 | ||
| R0403101-100M | mRNA Sequencing [Roche based] (100Million Paired-End Reads) using NovaSeq S4 | 17,483 | 26,224 | ||
| R0403108-100M | mRNA Sequencing [Roche based] (100Million Paired-End Reads) using NovaSeq S2 | 25,699 | 38,548 | ||
| D0121106-60X | Whole Genome Sequencing | Bacterial Whole Genome Sequencing (1gb data) using NovaSeq SP | 11,025 | 16,538 | |
| D0111102-30X | Human Whole Genome Sequencing (30X) using NovaSeq S4 | 88,961 | 1,33,442 | ||
| D0111102-60X | Human Whole Genome Sequencing (60X) using NovaSeq S4 | 1,68,227 | 2,52,341 | ||
| D0721106 | Metagenomic Sequencing | 16s rRNA (v3-v4) Microbiome Sequencing using NovaSeq SP | 2,953 | 4,430 | |
| D0721120 | 16s rRNA (v3-v4) Microbiome Sequencing using MiSeq | 8,581 | 12,872 | ||
| D0101106-10M | Shotgun Microbiome Sequencing (10M reads) using NovaSeq SP | 15,617 | 23,426 | ||
| D1701 | Targeted Sequencing | Targeted Sequencing (custom Panel) | Please contact for price | ||
| D0601103-50M | Epigenomic Sequencing | ChIP Sequening (50M reads) | 28,560 | 42,841 | |
| D08114 | Genotyping Array | Infinium GSA | 5,571 | 8,357 | |
| D09114 | Omni 2.5 | 23,561 | 35,342 | ||
| D10114 | EPIC Methylation | 37,320 | 55,980 | ||
| D16115 | Omni 5.0 | 37,825 | 56,738 | ||
| B18061 | Sequencing Only | illumina NovaSeq Sequencing | Please contact for price | ||
| B18062 | illumina MiSeq Sequencing | Please contact for price | |||
| |||||
| Service Code | Service Name | Service Description | Total cost per sample for Government organizations (INR) | Total cost per sample for Non Government organizations (INR) | |
| Analysis service** | core_datacenter@nibmg.ac.in | ||||
| DIARR001 | Genotyping array - Genotype calling - upto 500K probes - Illumina | Genotype call report - Per sample basis | 50 | 75 | |
| DIARR002 | Genotyping array - Genotype calling - more than 500K probes - Illumina | Genotype call report - Per sample basis | 250 | 375 | |
| DHOT0001 | Hotspot targeted sequening data analysis - Illumina | Annotated Hotspot mutation call - Only SNP/Indel - per sample | 500 | 750 | |
| DIEXSP01 | Exome sequencing data analysis - Somatic - Paired - Illumina | Annotated Somatic variant calls - Only SNP/Indel - per paired samples | 2,500 | 3,750 | |
| DIEXD002 | Exome sequencing data analysis - Illumina | Annotated Germline variant calls - Only SNP/Indel - per sample | 1,000 | 1,500 | |
| DIEXST03 | Exome sequencing data analysis - Somatic - Tumor only - Illumina | Annotated Somatic variant calls - Only SNP/Indel - per sample | 1,500 | 2,250 | |
| DIWGSP01 | Whole genome sequencing data analysis - Somatic - Paired - Illumina | Annotated Somatic variant calls - Only SNP/Indel - per paired sample | 7,500 | 11,250 | |
| DIWGD002 | Whole genome sequencing data analysis - Illumina | Annotated Germline variant calls - Only SNP/Indel - per sample | 3,500 | 5,250 | |
| DIRNAS001 | mRNAseq analysis (Input Fastq)- Single sample / group - Illumina | Gene expression count - per sample | 1,000 | 1,500 | |
| DIRNAP002 | mRNAseq analysis (Input Fastq) - Paired sample / group - Illumina | Differential expression analysis - per group | 2,500 | 3,750 | |
| DRNADE03 | Differential gene (mRNA) expression annotation analysis - Illumina | Pathway analysis - per job | 3,000 | 4,500 | |
| Consultation service | |||||
| DNGSC001 | Project consultation on NGS data analysis / study design | Per hour of consultation time | 5,000 | 7,500 | |
| DSTAT001 | Project consultation on statistical data analysis / study design | Per hour of consultation time | 5,000 | 7,500 | |
| Consulation and analysis | |||||
| DCUST001 | Custom analysis - Based on discussion and approval | Per day of custom analysis manpower time | 10,000 | 15,000 | |
| Training | |||||
| DTRAN001 | Training of programming, data management, server software installation and data analysis | Per month of stay and training in NIBMG, Guest house charges additional as per prevailing rate, maximum two months. | 80,000 | 1,20,000 | |
| ** includes hardware, software infrastructure, operational costs such as manpower and consultation | |||||
ZEBRAFISH FACILITY | |||||
| Service Code | Service Name | Service Description | Total cost per sample for Government organizations (INR) | Total cost per sample for Non Government organizations (INR) | |
| ZF2301 | Micro-Injections | 1.All needed items for Microinjections 2.Imaging 3.Maintenance and care | 5,000 | 7,500 | core_zebrafish@nibmg.ac.in |
| Anti-sense oligo/morpholino (one-cell stage) Or CRISPR (one-cell stage) Or Plasmid (one-cell stage) | |||||
| Or Cells (at 2dpf stage) | 4. Deep Phenotypic Study (Cryotome, staining, Slide preparation, imaging) (Including fixations medium) | 7,000 | 10,500 | ||
| (Needs to provide own reagent and IAEC approved) | 5.Confocal Imaging (per hour) | 500 | 750 | ||
| ZF2302 | Drug Screening | 1.Drug treatment 2.Dechorionation 3.Screening and imaging 4. Maintenance and care | 3,000 | 4,500 | |
| Needs to provide own drug and IAEC approved. | |||||
| 5. Confocal Imaging (per hour) | 500 | 750 | |||
| ZF2303 | Behavioural Study | 1.Using ZANTIKS System Analyser 2. Data analysis 3.Maintenance and care | 3,000 | 4,500 | |
| Experimental Conditions at 1dpf -14dpf larvae | |||||
| (IAEC approved) | |||||
| ZF2304 | IAEC approval | Protocol submission and execution, fees for IAEC, NIBMG | 10,000 | 15,000 | |
HUMAN MICROBIOME FACILITY | |||||
| Service Code | Service Name | Service Description | Total cost per sample for Government organizations (INR) | Total cost per sample for Non Government organizations (INR) | |
| HMF001 | Microbiome DNA/RNA isolation (Manual) | Minimum number of samples needed per user-50 | 2,100 | 3,150 | core_humanmicrobiome@nibmg.ac.in |
| HMF002 | Microbiome DNA/RNA isolation (Automated) | Minimum number of samples needed per user-192 | 2,100 | 3,150 | |
| HMF003 | 16S based PCR and Gel electrophoresis | Minimum number of samples needed per user-24 | 1,600 | 2,400 | |
| HMF004 | Project Consultation: Study Design and Data Analysis | Per hour consultation time | 5,000 | 7,500 | |
| HMF005* | 16S data analysis* | Minimum number of samples needed per user-10 (Deliverables: OTU/ASV, L2-L6, Core Diversity, Biome file) | 1000 | 1,500 | |
| HMF006* | Shotgun data analysis* | Based on discussion with facility-in-charge | |||
| HMF007 | Training in wet lab and dry lab modules | 2-3 weeks stay and training in NIBMG, Guest house charges additional as per prevailing rate | 30,000 | 45,000 | |
| * | The data analysis services will be carried out jointly with the data centre | ||||
BFDU FACILITIES | |||||
| Service Code | Service Name | Service Description | Total cost per sample for Government organizations (INR) | Total cost per sample for Non Government organizations (INR) | |
| BFDU001 | Bioplex | Bioplex use per plate | 14,135 | 20,502 | core_bfdu@nibmg.ac.in |
| BFDU002 | FACS analysis | FACS Analysis per hour | 2,500 | 3,500 | |
| BFDU003 | FACS sorting | FACS sorting per hour | 3,500 | 5,000 | |
| BFDU004 | DD-PCR | DD PCR per run; reagent kit not included | 1,500 | 2,000 | |
| BFDU005 | Ultracentrifuge | Ultracentrifuge run per hour; tube not included | 1,500 | 2,000 | |
IMAGING FACILITY | |||||
| Service Code | Service Name | Service Description | Total cost per sample for Government organizations (INR) | Total cost per sample for Non Government organizations (INR) | |
| CM001 | Confocal microscopy | Use of confocal microscope facility per hour | 500 | 750 | core_imaging@nibmg.ac.in |
PROTEOMICS FACILITY | |||||
| Service Code | Service Name | Service Description | Total cost per sample for Government organizations (INR) | Total cost per sample for Non Government organizations (INR) | |
| MS001 | Protein identification | Protein identification from single spot/band | 5,000 | 10,000 | core_proteomics@nibmg.ac.in |
| MS002 | Protein complexes (pull down) identification | 6,000 | 12,000 | ||
| MS003 | LC-MS/MS proteome analysis | LC-MS/MS proteome analysis (digested sample) [ORBITRAP MS/MS], Run 180 mins | 15,000 | 30,000 | |
| MS004 | LC-MS/MS proteome analysis (including sample preparation) [ORBITRAP MS/MS, Run 180 mins | 20,000 | 40,000 | ||
| For a combination of above mentioned services | core_combinationservice@nibmg.ac.in | ||||
