Profile
Research Interest:
Mass-spectrometry based proteomics and metabolomics: Chronic inflammatory diseases/disorders and resolution of inflammation
Selected Publications
1. Selenium-dependent metabolic reprogramming during inflammation and resolution. KORWAR AM, Hossain A, Lee TJ, Shay AE, Basrur V, Conlon K, Smith PB, Carlson BA, Salis HM, Patterson AD, Prabhu KS. (2021) J. Biol. Chem. Feb 10;296:100410. PMID: 33581115. https://pubmed.ncbi.nlm.nih.gov/33581115/
2. Comprehensive Quantification of Carboxymethyllysine-Modified Peptides in Human Plasma. KORWAR AM, Zhang Q. (2021) J. Am. Soc. Mass Spectrom. Mar 3;32(3):744-752. PMID: 33512994. https://pubmed.ncbi.nlm.nih.gov/33512994/
3. Development of Diagnostic Fragment Ion Library for Glycated Peptides of Human Serum Albumin: Targeted Quantification in Prediabetic, Diabetic, and Microalbuminuria Plasma by Parallel Reaction Monitoring, SWATH, and MSE. KORWAR AM, Vannuruswamy G, Jagadeeshaprasad MG, Jayaramaiah RH, Bhat S, Regin BS, Ramaswamy S, Giri AP, Mohan V, Balasubramanyam M, Kulkarni MJ. (2015) Mol. Cell. Proteomics. Aug;14(8):2150-9. PMID: 26023067. https://pubmed.ncbi.nlm.nih.gov/26023067/
4. Analysis of AGE modified proteins and RAGE expression in HER2/neu negative invasive ductal carcinoma. KORWAR AM, Bhonsle HS, Chougale AD, Kote SS, Gawai KR, Ghole VS, Koppikar CB, Kulkarni MJ. (2012) Biochem. Biophys. Res. Commun. Mar 16;419(3):490-4. PMID: 22366088. https://pubmed.ncbi.nlm.nih.gov/22366088/
5. Low plasma albumin levels are associated with increased plasma protein glycation and HbA1c in diabetes. Bhonsle HS, KORWAR AM, Kote SS, Golegaonkar SB, Chougale AD, Shaik ML, Dhande NL, Giri AP, Shelgikar KM, Boppana R, Kulkarni MJ. (2012) J. Proteome Res. Feb 3;11(2):1391-6. PMID: 22181049. https://pubmed.ncbi.nlm.nih.gov/22181049/
Publication Link:
https://www.ncbi.nlm.nih.gov/myncbi/1noBtL6LdITk-/bibliography/public/
Research
Our laboratory primary research is focused
on studying cellular metabolism implicated in chronic inflammatory diseases
(colon, pancreatic and oral cancer) and resolution of inflammation. In the
recent past, cellular metabolism has emerged as an integral process implicated
in generating key signaling molecules associated with various cellular
responses and processes including immune response, cell growth, apoptosis, and
redox homeostasis. Often, cancer cells adopt dramatic metabolic phenotype to
support proliferation and subsequent progression. Our lab is interested in
understanding the factors that influence/regulate proteome and metabolome remodeling
that facilitates such metabolic phenotype adaptations and develop potential
therapeutic avenues.
Our research
interest also includes type-I diabetic specific identification of novel
proteogenomic signatures associated with development of complications.
Secondly, to develop sensitive, accurate, and reproducible measurement methods
of biomolecules (especially proteins) to understand the pathophysiology and for
biomarker discovery.
CV
Education
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Year
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Degree
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University
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2012
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Ph.D.
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Savitribai Phule Pune University (Formerly University of Pune)
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2002
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M.Sc.
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Karnatak University
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1999
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B.Sc.
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Karnatak University
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Professional Appointments
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Postdoctoral Scholar. Centre for Translational Biomedical Research, University of North Carolina, Greensboro. USA [2019 to 2021].
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Postdoctoral Scholar. Department of Veterinary and Biomedical Sciences, The Pennsylvania State University. USA [2016 to 2019].
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Research Fellow. Department of Biological Sciences, Nanyang Technological University. Singapore [2015 to 2016]
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CSIR Research Associate. Biochemical Sciences Division, CSIR-National Chemical Laboratory. India [2013 to 2015]
Professional Membership
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Life member of the Indian Society for Mass Spectrometry (LM693) [From 2008]
- Life member of Proteomics Society of India (Reg. No. 553) [From 2022]
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Past member, American Society for Mass Spectrometry [From 2017 to 2021]
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Past member, American Society for Nutrition [From 2017 to 2019]
Honors and Awards
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NCL RF Gupta-Pardeshi-Sainani award for research scholars for best published research paper in biological sciences. Issuer: CSIR-National Chemical Laboratory Research Foundation. Pune, India-411008.
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Award of ‘Research Associate’ fellowship by CSIR, Government of India. April 2013
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Best poster and oral presentation at ‘International symposium- Proteomics beyond Ids’. CSIR- National Chemical Laboratory, Pune, India. November 2012.
Presentations
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The role of selenium in redox-dependent reversal of Warburg effect in macrophage polarization. KORWAR AM, Shay AE, Basrur V, Conlon K, Koo I, Smith PB, Patterson AD, Carlson BA, Prabhu KS. Nutrition 2018, the American Society for Nutrition's inaugural flagship meeting. June 9-12, 2018. Boston, MA (Poster presentation)
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Molecular profiling of breast cancer and identification of advanced glycation end products. KORWAR AM, Bhonsle HS, Kulkarni MJ International symposium- Proteomics beyond Ids’… Fourth Annual Meeting of Proteomics Society (India). November 22-24, 2012. CSIR-National Chemical Laboratory, Pune, India. (Poster presentation)
Publications
- Selenium-dependent metabolic reprogramming
during inflammation and resolution. KORWAR AM, Hossain A, Lee TJ, Shay AE,
Basrur V, Conlon K, Smith PB, Carlson BA, Salis HM, Patterson AD, Prabhu
KS. (2021) J. Biol. Chem. Feb 10;296:100410. PMID: 33581115. https://pubmed.ncbi.nlm.nih.gov/33581115/
- Comprehensive Quantification of
Carboxymethyllysine-Modified Peptides in Human Plasma. KORWAR AM, Zhang Q.
(2021) J. Am. Soc. Mass Spectrom. Mar 3;32(3):744-752. PMID:
33512994. https://pubmed.ncbi.nlm.nih.gov/33512994/
- Selenoproteome Identification in Inflamed
Murine Primary Bone Marrow-derived Macrophages by nano-LC Orbitrap Fusion
Tribrid Mass Spectrometry. KORWAR AM, Shay AE, Basrur V, Conlon K, Prabhu
KS. (2019) J. Am. Soc. Mass Spectrom. Jul;30(7):1276-1283. https://pubmed.ncbi.nlm.nih.gov/30972724/
- The Regulation of Pathways of Inflammation and
Resolution in Immune Cells and Cancer Stem Cells by Selenium. Diwakar BT,
KORWAR AM, Paulson RF, Prabhu KS. (2017) Adv. Cancer Res. 2017;136:153-
172. https://pubmed.ncbi.nlm.nih.gov/29054417/
- Targeted Quantification of the Glycated
Peptides of Human Serum Albumin. Vannuruswamy G, KORWAR AM,
Jagadeeshaprasad MG, Kulkarni MJ. (2017) Methods Mol. Biol.
2017;1619:403-416. https://pubmed.ncbi.nlm.nih.gov/28674900/
- Molecules with
O-acetyl Group Protect Protein Glycation by Acetylating Lysine Residues.
Vannuruswamy G, Jagadeeshaprasad MG, K. Kashinath, Suresh KK, Bhat SP,
KORWAR AM, Chougale AD, Boppana R, Reddy DS, Kulkarni MJ. (2016) RSC
Advances. 2016;6: 65572-65578. https://doi.org/10.1039/C6RA11313C
- Targeted
quantification of N-1-(carboxymethyl) valine and N-1-(carboxyethyl) valine
peptides of β- hemoglobin for better diagnostics in diabetes.
Jagadeeshaprasad MG, Batkulwar K, Meshram N, Tiwari S, KORWAR AM,
Unnikrishnan AG, Kulkarni MJ. (2016) Clinical Proteomics. 2016 Mar
29;13:7. https://pubmed.ncbi.nlm.nih.gov/27030792/
- Potential Dual Role of Eugenol in Inhibiting
Advanced Glycation End Products in Diabetes: Proteomic and Mechanistic
Insights. Singh P, Jayaramaiah RH, Agawane SB, Vannuruswamy G, KORWAR AM,
Anand A, Dhaygude VS, Shaikh ML, Joshi RS, Boppana R, Kulkarni MJ,
Thulasiram HV, Giri AP. (2016) Scientific Reports. 2016 Jan 7;6:18798. https://pubmed.ncbi.nlm.nih.gov/26739611/
- Development of diagnostic fragment ion library
for AGE modified peptides of Human serum albumin: Their use in targeted
quantification using PRM, SWATH and MS. KORWAR AM, Vannuruswamy G,
Jagadeeshaprasad MG, Jayaramaiah RH, Bhat S, Regin BS, Ramaswamy S, Giri
AP, Mohan V, Balasubramanyam M, Kulkarni MJ. (2015) Mol. Cell. Proteomics
2015 Aug; 14(8):2150-9. https://pubmed.ncbi.nlm.nih.gov/26023067/
- Recent development of plant products with
anti-glycation activity: a review Chinchansure AA, KORWAR AM, Kulkarni MJ
and Joshi SP. (2015) RSC Advances. 2015,5, 31113-31138. DOI:
10.1039/C4RA14211J. https://doi.org/10.1039/C4RA14211J
- Proteomic profiling and interactome analysis
of ER-positive/HER2/neu negative invasive ductal carcinoma of the breast:
towards proteomics biomarkers. KORWAR AM, Bhonsle HS, Ghole VS, Gawai KR,
Koppikar CB, Kulkarni MJ. (2013) OMICS: A journal of Integrative Biology.
2013; 17(1):27-40. https://pubmed.ncbi.nlm.nih.gov/23301641/
- Proteomic study reveals downregulation of
apolipoprotein A1 in plasma of poorly controlled diabetes: a pilot study.
Bhonsle HS, KORWAR AM, Chougale AD, Kote SS, Dhande NL, Shelgikar KM,
Kulkarni MJ. (2013) Molecular Medicine Reports. 2013; 7(2):495-8. https://pubmed.ncbi.nlm.nih.gov/23232761/
- Glycated proteome: from reaction to
intervention. Kulkarni MJ, KORWAR AM, Mary S, Bhonsle HS, Giri AP. (2013)
Proteomics Clin. Appl. 2013; 7(1-2):155-70. https://pubmed.ncbi.nlm.nih.gov/23184864/
- Analysis of AGE modified proteins and RAGE
expression in HER2/ neu negative invasive ductal carcinoma. Korwar AM,
Bhonsle HS, Chougale AD, Kote SS, Gawai KR, Ghole VS, Koppikar CB,
Kulkarni MJ. (2012) Biochem Biophys Res Commun. 2012; 419(3):490- 4. https://pubmed.ncbi.nlm.nih.gov/23184864/
- "Zoom-ln"--A targeted database
search for identification of glycation modifications analyzed by
untargeted tandem mass spectrometry. Bhonsle HS, Korwar AM, Kesavan SK,
Bhosale SD, Bansode SB, Kulkarni MJ. (2012) Eur. J. Mass Spectrom.
(Chichester, Eng). 2012; 18(6):475-81. https://pubmed.ncbi.nlm.nih.gov/23654192/
- Low plasma albumin levels are associated with
increased plasma protein glycation and HbA1c in diabetes. Bhonsle HS,
Korwar AM, Kote SS, Golegaonkar SB, Chougale AD, Shaik ML, Dhande NL, Giri
AP, Shelgikar KM, Boppana R, Kulkarni MJ. (2012) J. Proteome Res. 2012;
11(2):1391- 6. https://pubmed.ncbi.nlm.nih.gov/22181049/
Lab members
PhD Student
Ms. Anjali Gupta. CSIR-JRF.
