Faculty Members

Research

Monogenic Disorder

Thalassemia syndrome or hemoglobinopathy is the most frequent monogenic disorder in India. Regular blood transfusion is available option for managing this genetic disorder.

It has been observed that there is remarkable phenotype heterogeneity among the thalassemia patients with same HBB genotype. Recently, we have showed that HbE/β thalassemia with γGglobin-158 C/T [HBG2 c.-211 C>T ; NC_ 000011.9: ] polymorphism may be treated with hydroxy urea and can lower the need of blood transfusion.

We did a family-based whole exome sequence (WES) study and figure out the probable modifier genes that are responsible for making severe phenotype among the HbE/β thalassemia patients (Unpublished). Currently, we are doing WES in a larger patient group for validating the result of family based study.

condition of Thalassemia depends on the degree of ineffective erythropoiesis which is due to delayed maturation of erythroblasts. It has been reported that the bone marrow of beta thalassemia patients contains 5-6 times the number of erythroid precursors than normal healthy individuals with accelerated apoptosis and decreased maturation. At present we are trying to understand molecular pathway involved in the ineffective erythropoiesis and drug target.

One of the obstructions for the thalassemia prevention is the non-availability of any simple field-based method or kit for carrier screening. Only available method is the HPLC based method, which limits the access of the large population of India for carrier detection program. Under the funding support from BIRAC, DBT a field based simple carrier screening kit has been developed. Accordingly, patent has been filed recently to the Indian patent office.

Cancer Immunology:

It is now well established that immune system has a pivotal role in cancer progression. Different immune cells, receptors, checkpoint inhibitors along with other stromal cells control the proliferation of clone of cancers cells. Presently our group is working to understand the cross talk between immune cells: Monocyte /Macrophage and breast cancer cells. We are also looking for soluble markers for breast cancer.

It has been known for long time that Innate immune receptor -TLR3, locating inside the endosomal compartment of the cells, promote apoptosis. Accordingly, ligands of TLR3 had been used as adjuvant therapy in several clinical trials. Our group first time discovered that TLR3 also present on cell surface apart from endosomal compartment and induce cellular proliferation instead of apoptosis.

It has been known earlier that TLR3 use TRIF mediated signalling pathway to induce apoptosis. Very recently our group has discovered that when TLR3 is present on the surface of the cancer cell cells, uses different signaling pathway involving MyD88 - NFkB mediated signalling cascade to influence cellular proliferation.

Our group has already shown the diagnostic and prognostic importance of TLR2 and TLR9 in breast and cervical cancer. It has already been showed the role of different proteases in epithelial to mesenchymal transition (EMT) for breast cancer metastasis. Accordingly, our group has showed that TLR 4 expresses in non-immune ductal epithelial cells of the breast cancer and can be responsible for the cellular invasiveness (work is already archived in BIORXIV)

Rare disorders

Lot of Indian people are suffering from different types of inherited disorders and their frequency in the population lower than the established monogenic or polygenic dieses. As per WHO definitions, rare disease as often debilitating lifelong disease or disorder with a prevalence of 1 or less, per 1000 population. Major problems with rare disorders that they are muti-genic origin and variants are not common. Thus, we have initiated to do case by case approach for identifying indigenous gene and variants using whole exome sequencing method. Recently we have discovered CHRNE: NG_008029.2:g.6441_6454dup (Homozygous) is responsible inherited Myasthenia Gravis (unpublished data) in a Bengali patient .

CV

Present Position: ASSOCIATE DIRECTOR
National Institute of Biomedical Genomics
Kalyani, West Bengal, India
ab3@nibmg.ac.in

Department of Zoology
The University of Burdwan
Burdwan -713104
e-mail: abasu@zoo.buruniv.ac.in

Highest Degree :
Ph.D  (2005) : National Institute of Health and Family Welfare, New Delhi. [Degree: The University of Burdwan]

Post Doctoral Fellow (2006-07):
Department of Cell Biology and Physiology, School of Medicine, University of New Mexico, USA

Earlier positions Held
Professor (30/07/2017 to 08/11/21) : Dept. of Zoology, The University of Burdwan, WB. India

Head of the Department (28/02/2013 – 27/02/ 2015) : Dept. of Zoology, The University of Burdwan, WB. India

Associate professor (26/12/2010- 29/07/ 2017): Dept. of Zoology, The University of Burdwan, WB. India Reader (26/12/2007 to 25/12/2010) : Dept. of Zoology, The University of Burdwan, WB. India

Member-Editorial Board:  Scientific Reports (NPG),

Professional Memberships:

Elected executive member of the Indian Society of Cell Biology (2017-2019)
Life Member of The Indian Society of Cell Biology
Life Member of Society of Biological Chemists, India
Life Member Indian Science Congress Association
Life Member The Society for Biotechnologists (India)
Life Member of Society of Reproductive Biology and Comparative Endocrinology

Invited/Special lectures:

• Delivered invited talk on “Thalassemia or Hemoglobinopathy: Understanding the Clinical Heterogeneity and genetic background” Organized by Kansas City VA Medical Centre, USA , 16 September 2021
• Delivered invited talk on “Build your own Cocoon with wish and will”, National Webinar on Health and Health Care in the Current Scenario COVID19, 08/05/2020, organised by International Quality Assurance Cell, The Maharaja Sayajirao University of Baroda
• Delivered invited talk on “Phenotype heterogeneity of thalassemia syndrome and search of modifiers”, National Seminar on Advancement of Biology In The 21st Century, 29/02/2020, Dept. Of Zoology, Viswa Bharati
• Delivered invited talk on “Human genetical disorders”, DST INSPIRE Internship Science Camp 2020, 04/01/2020, Durg, Chhattisgarh
• Delivered invited talk on “Phenotype genotype enigma of thalassemia syndrome in Indian population”, 19th All India Congress of Genetics and Genomics Conference organized by CSIR-IICB and Archana Sharma Foundation of Calcutta, 2-4/12/2019, CSIR-IICB, Kolkata
• Delivered invited talk on “Development of Novel Universal Field based Thalassemia Carrier Screening Kit- Tool for hemoglobinopathy prevention”, 5th. International conference on Transnational Research -ICCTR 2019 (November 7-9. 2019), 7-9/11/2019, NCCS, Pune
• Delivered invited talk on “Understanding the Genotype- Phenotypic heterogeneity of Thalassemia Syndrome”, International Conference on Recent Advances in Informatics, Communication, Management, Health and Applied Sciences, 2-4/02/2019, Brainware University, Kolkata
• Delivered invited talk on “Revelation of major inherited disorders in Indian population”, Invited talk on Thalassemia Genetics, 08/09/2018, IISER-Kolkata
• Delivered invited talk on “Application of immunohistochemistry and fluorescence microscopy in cancer detection”, Optical Microscopy with Image Analyzer and Its Application, 28/03/2018, Sophisticated Analytical Instrument Facility (SAIF), NEHU, Shilong
• Delivered invited talk on “Questioning of the canonical signaling of toll-like receptor in tumor micro-environment”, 37 th IACR Convention 2018, 23-25/2/2018, Bose Institute, Unified Academic Campus, Kolkata
• Chair a session in the “8th East Zonal Oncology Symposium”, at Saroj Gupta Cancer Centre & Research Institute, Thakurpukur, 21st January, 2017
• Chair a session in the “XL All India Cell Biology Conference and International Symposium on Functional Genomics and Epigenomics”, organized by Indian Society of Cell Biology and Jiwaji University, Gwalior ,November 17-19,2016
• Delivered invited talk on, “Saga of Thalassemia syndrome in Indian population and researchable issues”, International Conference celebrating 100 years of Zoology, University of Calcutta (INTZOOCON 2018), 1-3/02/2018, Calcutta University
• Delivered invited talk on “The spectrum of globin chain mutation in genotype- phenotype heterogeneity of thalassemia syndrome in Indian population: Modifier Gene and Clinical Management”, XI AAPI Global Health Care Summit -2017, 29/12/2017, JW Marriott, Kolkata
• Delivered invited talk on “Phenotype heterogeneity of thalassemia syndrome and possible management”, 3rd International Conference on Translational Research, 25/09/2017, Amity University, Kolkata
• Delivered invited talk on “Role of Toll-like receptors in tumor micro-environment and cancer progression”, 4th International Conference on Angiogenesis Research Organized by Saroj Gupta Cancer Centre & Research Institute, Thakurpukur, 21/09/2017, Saroj Gupta Cancer Centre & Research Institute, Thakurpukur
• Delivered invited talk on “Role of TLRs in breast cancer progression”, 2nd International Conference on Translational Research: Applications in Human Health & Agriculture, 14-16/10/2016, KIIT University and NCCS Pune
• Delivered invited talk on “Epithelial to mesenchymal transition(EMT) and toll-like receptors (TLRs) in cancer progression” International Conference on Translational Research Jointly Organized by Indian Society of Translational Research Bose Institute, Kolkata, 16-18/04/2016, Bose Institute, Kolkata
• Delivered invited talk on “Toll-like receptors for the treatment and management of cervical and breast cancer”, National Symposium on Comparative Endocrinology and Reproductive biology, 1-3/10/2015, Department of Zoology, Visva-Bharati, Santiniketan
• Delivered invited talk on “Crosstalk between TLR4 and proteases for epithelial to mesenchymal transition in breast cancer progression”, 3rd. International Conference on Angiogenesis organized, 23-25/09/2015, Sastra University
• Delivered invited talk on “Toll like receptor signaling can promote breast cancer progression through epithelial to mesenchymal transition”, National Seminar on “Life and Life Processes: Sustainable Development, 19-21/02/2015, Goa University
• Delivered invited talk on “Whether Toll-like receptor-3 signaling suppress or induce cancer cell proliferation?”, 2nd. International Conference on Angiogenesis, 2-4/02/2014, National Center for Cell Science and KIIT University
• Delivered invited talk on “Compound mutations in β-Thalassemia Patients of West Bengal Population”, 100th. Session of Indian Science Congress, 3-7/01/2013, Kolkata
• Delivered invited talk on “Plasminogen activator inhibitor stimulates angiogenesis in oxygen induce mice retinopathy”, XXXII Conference of Indian Society of Cell Biology (ISCB) and Symposium on “Stem Cells and Pattern Formation”, 4-6/12/2008, ISCB and MACS- Agharkar Research Institute, Pune
• Delivered invited talk on “Diabetics and retinal angiogenesis: Role of proteinases”, Symposium of XXVI SRBCE, 17-19/01/2008, Tiruchirapalli

Present ongoing Project

A Genetic Algorithm-Based Targeted Approach for Understanding the Phenotypic Heterogeneity of Thalassemia Syndromes in Northern and Eastern Indian Population. [Multicentre project participated by PGIMER, Chandigarh & The University of Burdwan, Burdwan; Burdwan Medical College, Institute of Child Health, Kolkata] – Act as project coordinator as well PI (DBT-GoI; 2018 to Continue)

• Study on the role of TLR4 signalling in breast cancer progression  (DBT-GoI; 2018 to 2021)
• Development of field based rapid hemoglobinopathy - Thalassemia carrier screening kit (BIRAC, 2018-2020)
• Study on the effect of TLR-3 and TLR-9 on breast cancer (SERB, DST, GoI; 2016-2019)
• Study on the Mutation profile of Beta Globin gene and sequence homology among anthropoids ( Govt of WB Science and Technology, 2016-2019)
• The simultaneous inhibition of plasminogen activator along with vascular endothelial growth factor (VEGF) in controlling angiogenesis SERB, DST, GoI; 2011-2014)
• Validation of messenger RNA based diagnosis and antigen-based diagnosis of the angiogenesis markers (UGC, 2009 to 2012).

Conference / Training Program Organized as Convenor/Organizing Secretary

• Organized International Symposium on Human Genetics: Population and Disease and Workshop on Thalassemia, 24-25 th. August, 2018
• Organized National Conference on Advances of Life Sciences and Radiation Biology and Seminar of IACR West Bengal Chapter, 17-18th February, 2017
• Organized International Symposium on Genetic Analysis: Translational and Developmental (iNSGTD) and Annual Meeting of Society for Biotechnologists (India), November 21 -23’2014
• One day workshop cum seminar on “Frontiers in Molecular Biology and Workshop on Basic Flowcytometry” held on July 9’2012;
• A two days “Science Academies’ Lecture Workshop on Progress and Prospects of Biological sciences”, as Jt. Convener Sponsored by Indian Academy of Sciences (IASc, INSA and NASI), From August 16 -17’2011
• A two week workshop on Molecular Biological Techniques as Course Director, held on September 7-19'2009,

Publications

• Ghosh D, Panja A, et al, Basu A. Drug repurposing: Hydroxyurea therapy improves the transfusion-free interval in HbE/beta-thalassemia–major patients with XmnI polymorphism. Genet Test Mol Biomarkers; 25 (8) 563-70, 2021.
• Singh A, Bondhopadhyay A, Mukherjee M,  Basu A.  Toll-Like Receptor 9 Expression Levels in Breast Carcinoma Correlate with Improved Overall Survival in Patients Treated with Neoadjuvant Chemotherapy and Could Serve as a Prognostic Marker. Genet Test Mol Biomarkers, 25(1):12-19; 2021.
•  Singh A, Devkar R and Basu A. Myeloid Differentiation Primary Response 88–Cyclin D1 Signaling in Breast Cancer Cells Regulates Toll-Like Receptor 3-Mediated Cell Proliferation. Front. Oncol. 10:1780, 2020.
•  Chatterjee S, Patra D, Chakraborti U, Basu A., et al. Association of p38MAPK-p53-Fas aggregation in S-allyl cysteine mediated regulation of hepatocarcinoma. Environmental Toxicology. 2019;1–13
• Panja A, et al., Basu A.  Cross-Sectional Study for the Detection of Mutations in the Beta-Globin Gene Among Patients with Hemoglobinopathies in the Bengali.  Genet Test Mol Biomarker. 21:39-45, 2017.
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• Moirangthem A, Bondhopadhyay B, Mukherjee M,  Bondhopadhyay A, Mukherjee M, Konar K, Bhattachrya S,   Basu A. Simultaneous knockdown of uPA and MMP9 can reduce breast cancer progression by increasing cell-cell adhesion and modulating EMT genes.  Scientific Reports 6, 21903, 2016
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• Panja A, Chowdhury P, Basu A. Hb Midnapore a novel  variant with a structure abnormality [β53 (D4) Ala >Val HBB:c.161C>T] associating  with  (HBB:c 92+5 G>C) found in Bengali Indian family Hemoglobin. 40:300-303, 2016.
• Kundan TC, Bondhopadhyay B, Keshav K, Sahoo SK, Basu A, et al..,  A novel zinc(II) and hydrogen sulphate selective fluorescent “turn-on” chemosensor based on isonicotiamide: INHIBIT type's logic gate and application in cancer cell imaging. Analyst, 2016, 141, 1814-1821.
• PK Chowdhury, M Saha,  Basu A, D Chowdhury.  Profile of Iron Overload in Nontransfusion Dependent Hb E Beta Thalassaemia Patients- Is It Different?  Blood, 126, 4557 – 4557, 2015 [IF  10.5]  
• Bondhopadhyay, B, Moirangthem A, Basu A. Innate adjuvant receptor Toll –like receptor 3 can promote breast cancer through cell surface Tumor Biology –36:1261–1271,2015 
• Ghosh A, Moirangthem A, Dalui R, Ghosh TK, Bandyopadhyay A, Dasgupta A, Banerjee U, Basu A. Expression of Matrix Metalloproteinase 2 and 9 in cervical intraepithelial neoplasia and cervical carcinoma among different age groups of premenopausal and postmenopausal  women. J of Cancer Research and Clinical Oncology, 140:1585-1593 2014)
• Ghosh A, Dasgupta A, Bandyopadhyay A, Ghosh TK, Dalui R, Biswas S,  Banerjee U, Basu A. A  study of the expression and localization of Toll-Like Receptors 2 and 9 in different grades of cervical intraepithelial neoplasia and squamous cell carcinoma. Exp. Mol. Pathol  99: 720–724, 2015.  
• D Mahajan, N Khairnar, B Bondhopadhyay, SK Sahoo, Basu A et al., A highly selective fluorescent ‘turn-on’chemosensor for Hg 2+ based on a phthalazin-hydrazone derivative and its application in human cervical cancer cell imaging. New Journal of Chemistry, 39, 3071-3076, 2015.
• Rout JK, Dasgupta A, Singh O, Banerjee U Basu A. Association of single-nucleotide polymorphism of cholecystokinin receptor A gene with schizophrenia in an Eastern Indian population. Indian J Psychiatry.  2015 ;57(3):267-71.
• D Sharma, A Moirangthem, SK Sahoo, Anupam Basu, et al. Anion selective chromogenic and fluorogenicchemosensor and its application in breast cancer live cell imaging. RSC Advances 4, 41446-41452, 2014
• Tayade K, Sahoo S, Bondhopadhyay, B ,Bharadaj V, Singh N and Basu A et al. . Highly selective turn-on fluorescent sensor for nanomolar detection of biologically important Zn2+ based on isonicotinohydrazide derivative: Application in cellular imaging. Biosensors and Biolectronics. 61, 429-433, 2014
• Tayade K, Bondhopadhyay, B , and Basu A. et al. A novel urea-linked dipodal naphthalene-based fluorescent sensor for Hg(II) and its application in live cell imaging. Talanta.  122:16-22 , 2014
• Sen S, Mukherjee T, Chattopadhyay B, Moirangthem A, Basu A, et al Efficient and Convenient Methods for Synthesis of Some Phthalazine Derivatives and Their Evaluation of Cytotoxicity. Synthetic Communications 44:  847-857 (2014)
• H. Paul, T. Mukherjee, S. Sarkar, T.K. Mandal, A. Moirangthem A Basu A, et al. Ruthenium(II) complexes of pyrrol-azo ligands: cytotoxicity, interaction with calf thymus DNA and bovine serum albumin. Journal of Coordination Chemistry 66 (15), 2747-2764, 2013
• Sen S, Mukherjee T, Chattopadhyay B, Moirangthem A, Basu A, et al.  A water soluble Al3+ selective colorimetric and fluorescent turn-on chemosensor and its application in living cell imaging. Analyst. 137, 3975–3981(2012) 
• Sen S, Sarkar S, ChattopadhyayB,Moirangthem A, Basu A et al. A ratiometric fluorescent chemosensor for iron: discrimination of Fe²⁺ and Fe³⁺ and living cell application.  Analyst 137, 3335-3342; (2012) 
• 39. SK Sahoo, D Sharma, A Moirangthem, A et al., Basu A. Pyridoxal derived chemosensor for chromogenic sensing of Cu²⁺ and fluorogenic sensing of Fe³⁺ in semi-aqueous medium. Journal of Luminescence, 172, 297–303, 2016

• su A, McGuire P, Menicucci G, Maestes J, Das A.  Plasminogen activator inhibitor -1(PAI-1) Facilitates Angiogenesis in Experimental Retinopathy. IOVS  Oct;50(10):4974-81.(2009)
• Basu A, Maitra SK and Shrivastav TG. Development of dual-enzyme-based simultaneous immunoassay for measurement of progesterone and human chorionic gonadotropin. Analytical Biochemistry 366; 175–181: (2007).
• Basu A, Maitra SK and Shrivastav TG. A Direct Antigen Heterologous Enzyme Immunoassay    for Progesterone in Serum without Using Displacer. Steroid 71(3) 222-230; (2006).
• Basu A,Shrivastav TG and KariyaKP. Preparation of enzyme conjugate through adipic acid dihydrazide as linker and its use in immunoassay. Clin.Chem. 49(8), 1410-1412;   (2003).
• Basu A, Nara S, Chaube S. Rangari K, KariyaKP and Shrivastav TG. The influence of spacer containing enzyme conjugate on the sensitivity and specificity of enzyme immunoassays    for hapten. Clinica Chimica Acta, 366 (1-2),287-292; (2006)