Structural and functional integration of monogenic as well as rare genetic, disorders
Role of innate immunity in the progression of breast and cervical cancer
Singh A, Devkar R and Basu A. Myeloid Differentiation Primary Response 88–Cyclin D1 Signaling in Breast Cancer Cells Regulates Toll-Like Receptor 3-Mediated Cell Proliferation. Front. Oncol. 10:1780, 2020
Ghosh A, Moirangthem A, Dalui R, Ghosh TK, Bandyopadhyay A, Dasgupta A, Banerjee U, Basu A. Expression of Matrix Metalloproteinase 2 and 9 in cervical intraepithelial neoplasia and cervical carcinoma among different age groups of premenopausal and postmenopausal women. J of Cancer Research and Clinical Oncology, 140:1585-1593 2014
Chatterjee S, Patra D, Chakraborti U, Basu A., et al. Association of p38MAPK-p53-Fas aggregation in S-allyl cysteine mediated regulation of hepatocarcinoma. Environmental Toxicology. 2019;1–13
Ghosh D, Panja A, et al, Basu A. Drug repurposing: Hydroxyurea therapy improves the transfusion-free interval in HbE/beta-thalassemia–major patients with XmnI polymorphism. Genet Test Mol Biomarkers; 25 (8) 563-70, 2021.
PK Chowdhury, M Saha, Basu A, D Chowdhury. Profile of Iron Overload in Nontransfusion Dependent Hb E Beta Thalassaemia Patients- Is It Different? Blood, 126, 4557 – 4557, 2015
Thalassemia syndrome or hemoglobinopathy is the most frequent monogenic disorder in India. Regular blood transfusion is available option for managing this genetic disorder.
It has been observed that there is remarkable phenotype heterogeneity among the thalassemia patients with same HBB genotype. Recently, we have showed that HbE/β thalassemia with γGglobin-158 C/T [HBG2 c.-211 C>T ; NC_ 000011.9: ] polymorphism may be treated with hydroxy urea and can lower the need of blood transfusion.
We did a family-based whole exome sequence (WES) study and figure out the probable modifier genes that are responsible for making severe phenotype among the HbE/β thalassemia patients (Unpublished). Currently, we are doing WES in a larger patient group for validating the result of family based study.Understanding the ineffective erythropoiesis and clinical complexity of Thalassemia:
condition of Thalassemia depends on the degree of ineffective erythropoiesis which is due to delayed maturation of erythroblasts. It has been reported that the bone marrow of beta thalassemia patients contains 5-6 times the number of erythroid precursors than normal healthy individuals with accelerated apoptosis and decreased maturation. At present we are trying to understand molecular pathway involved in the ineffective erythropoiesis and drug target.Development of field-based thalassemia carrier detection kit and protype ready for Technology transfer to Industry (Patent submitted)
One of the obstructions for the thalassemia prevention is the non-availability of any simple field-based method or kit for carrier screening. Only available method is the HPLC based method, which limits the access of the large population of India for carrier detection program. Under the funding support from BIRAC, DBT a field based simple carrier screening kit has been developed. Accordingly, patent has been filed recently to the Indian patent office.
It is now well established that immune system has a pivotal role in cancer progression. Different immune cells, receptors, checkpoint inhibitors along with other stromal cells control the proliferation of clone of cancers cells. Presently our group is working to understand the cross talk between immune cells: Monocyte /Macrophage and breast cancer cells. We are also looking for soluble markers for breast cancer.Discovery of the Surface Localization of Toll -like Receptor 3 in Cancer Cell
It has been known for long time that Innate immune receptor -TLR3, locating inside the endosomal compartment of the cells, promote apoptosis. Accordingly, ligands of TLR3 had been used as adjuvant therapy in several clinical trials. Our group first time discovered that TLR3 also present on cell surface apart from endosomal compartment and induce cellular proliferation instead of apoptosis.Discovery of cell surface mediated MyD88 -TLR3 signalling cascade
It has been known earlier that TLR3 use TRIF mediated signalling pathway to induce apoptosis. Very recently our group has discovered that when TLR3 is present on the surface of the cancer cell cells, uses different signaling pathway involving MyD88 - NFkB mediated signalling cascade to influence cellular proliferation.Clinical relevance of TLR2, TRR4 and TLR 9 in cervical and breast cancer
Our group has already shown the diagnostic and prognostic importance of TLR2 and TLR9 in breast and cervical cancer. It has already been showed the role of different proteases in epithelial to mesenchymal transition (EMT) for breast cancer metastasis. Accordingly, our group has showed that TLR 4 expresses in non-immune ductal epithelial cells of the breast cancer and can be responsible for the cellular invasiveness (work is already archived in BIORXIV)
Lot of Indian people are suffering from different types of inherited disorders and their frequency in the population lower than the established monogenic or polygenic dieses. As per WHO definitions, rare disease as often debilitating lifelong disease or disorder with a prevalence of 1 or less, per 1000 population. Major problems with rare disorders that they are muti-genic origin and variants are not common. Thus, we have initiated to do case by case approach for identifying indigenous gene and variants using whole exome sequencing method. Recently we have discovered CHRNE: NG_008029.2:g.6441_6454dup (Homozygous) is responsible inherited Myasthenia Gravis (unpublished data) in a Bengali patient .
Present Position: ASSOCIATE DIRECTOR
National Institute of Biomedical Genomics
Kalyani, West Bengal, India
Department of Zoology
The University of Burdwan
Highest Degree :
Ph.D (2005) : National Institute of Health and Family Welfare, New Delhi. [Degree: The University of Burdwan]
Post Doctoral Fellow (2006-07):
Department of Cell Biology and Physiology, School of Medicine, University of New Mexico, USA
Earlier positions Held
Professor (30/07/2017 to 08/11/21) : Dept. of Zoology, The University of Burdwan, WB. India
Head of the Department (28/02/2013 – 27/02/ 2015) : Dept. of Zoology, The University of Burdwan, WB. India
Associate professor (26/12/2010- 29/07/ 2017): Dept. of Zoology, The University of Burdwan, WB. India Reader (26/12/2007 to 25/12/2010) : Dept. of Zoology, The University of Burdwan, WB. India
Member-Editorial Board: Scientific Reports (NPG),
Elected executive member of the Indian Society of Cell Biology (2017-2019)
Life Member of The Indian Society of Cell Biology
Life Member of Society of Biological Chemists, India
Life Member Indian Science Congress Association
Life Member The Society for Biotechnologists (India)
Life Member of Society of Reproductive Biology and Comparative Endocrinology
A Genetic Algorithm-Based Targeted Approach for Understanding the Phenotypic Heterogeneity of Thalassemia Syndromes in Northern and Eastern Indian Population. [Multicentre project participated by PGIMER, Chandigarh & The University of Burdwan, Burdwan; Burdwan Medical College, Institute of Child Health, Kolkata] – Act as project coordinator as well PI (DBT-GoI; 2018 to Continue)